In this experiment badgers (Meles meles), which were caught as part of an on-going population study, were either transported to a central site prior to blood sampling or blood was collected at their site of capture. Using the leukocyte coping capacity (LCC) test kit, the ability of leukocytes to produce reactive oxygen species (ROS) in vitro was assessed.

Transported animals had a significantly lower leukocyte coping capacity score (LCC) and showed changes in leukocyte composition that were indicative of stress. The study concluded that the stress of transport reduced LCC in badgers and that LCC serves as a quantitative measure of stress. Potential applications of this method are discussed.

MCLAREN, G., MACDONALD, D.W., GEORGIOU, C., NEWMAN, C. and MIAN, R. (2003) Leukocyte coping capacity: a novel technique for measuring the stress response in vertebrates Experimental Physiology 88 (4) pp. 541-546 (ISSN: 0958 0607).

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This study assessed the effect of exposure of volunteers to a mild, brief, psychologically stressful event, on the functional ability of leukocytes to produce reactive oxygen species in vitro.

Volunteers operated a car electric window and adjusted it to 4 pre-determined positions. Between each operation the mechanism’s polarity was covertly altered (group B) or remained unaltered (group A). For each treatment group 10 different subjects provided capillary blood samples pre- and post-stressor.

Using the leukocyte coping capacity (LCC) test kit, the ability of leukocytes to produce reactive oxygen species (ROS) in vitro was assessed. ROS release differed significantly at 10 min post-stressor between treatment groups, suggesting exposure to acute psychological stress leads to a reduced ability to respond to bacterial challenge.

SHELTON-RAYNER G, MIAN R, CHANDLER S, ROBERTSON D, MACDONALD DW (2011) Quantifying transient psychological stress using a novel technique: changes to PMA-induced leukocyte production of ROS in vitro. Int J Occup Safety & Ergonomics (JOSE) 17 (1), 3-13.

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This study, comprising of two phases, suggests that the immune system follows a similar trend. In phase 1: 15 healthy subjects provided capillary blood samples before and after completing three basic tasks using, in turn, two automotive touch screen interfaces (Interface 1 antecedent version, Interface 2—improved version).

Using the leukocyte coping capacity (LCC) test kit, the ability of leukocytes to produce reactive oxygen species in vitro was assessed. Significant differences in leukocyte activity were shown between treatment groups, where the greatest post-test decrease occurred after using Interface 1.

Phase 2: a randomly selected sub-group (n = 4) underwent weekly repeat testing using both interfaces. Significant differences in post-test leukocyte reactivity were exhibited between test weeks for each interface- the magnitude of response decreasing with successive exposure.

SHELTON-RAYNER G, CHANDLER S, ROBERTSON D, MIAN R & MACDONALD DW (2010) Leukocyte reactivity as an objective means of quantifying mental loading during ergonomic evaluation. Cellular Immunology 263, 22–30

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